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In vivo studies

Orthotopic animal model

The subcutaneous nude mouse xenograft model is widely used for in vivo screening. It provides information on primary tumor response, but because these subcutaneously implanted tumors growth in different microenvironment. In recent years, orthotopic models have been devised in which human tumors are implanted directly into the appropriate organ or site of origin in the laboratory animal. The orthotopic model of human cancer cells grafted into the nude mice that would mimic the clinical development of human cancer.

Tumor model Injection/Implantation site Cell lines
Intracerebral U-87MG, U-138MG, U-251MG, U-373MG
Intracerebral/ Intracarotid MDA-MB-435, MDA-MB-231, MCF-7
Intracerebral/ Intracarotid PC14PE6, H460, A549, H23, H1299
Mammary fatpad MDA-MB-435, MDA-MB-231, MCF-7
Left lung parenchyma PC14PE6, H460
intravenous Raji
Cecal wall H29,COLO205
Mammary fatpad MDA-MB-435, MDA-MB-435 Br1

Br1a : Variant brain metastatic cell line derived by sequential injection. Number refers to the generation.

Subcutaneous xenograft animal model

Typical IRCR Timeline
Typical IRCR Timeline
Subcutaneous Models
Tumor model Cell lines
Globlstoma U-87MG, U-118MG, U-138MG, U-251MG, U-373MG, SW 1088, M059J, LN229
Breast Cancer BT-474; MDA-MB-435, MDA-MB-231, MCF-7, MDA-MB-453, MDA-MB-468
Lung Cancer PC14PE6, H460, H23, H226, H 358, H1299, A549
Lymphoma Raji, Ramos, Bjab, Daudi
Colorectal H29, COLO205, HCT-166
Pancreatic BXPC-3, CFPAC-1, MiaPaCa, PANC-1
Bladder HT-1197, HT-1376, J82
Prostate 22Rv1, DU 145, LNCaP.FGC, PC-3
Kidney 786-0
Stomach MKN-45
Representative IRCR Results
Representative IRCR Results 1 Representative IRCR Results 2