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In vitro studies

Proliferation Assay

Monolayer Cell Proliferation Assay

Monolayer Cell Proliferation Assay Cell proliferation assay allows to determine the number of cells that are growing in the absence or presence of certain agents. A specified number of cells is seeded in the wells of 96-well plates and incubated for desired time periods (e.g., 48 and 72 hr). Proliferation rates are determined by non-radioactive/colorimetric assay methods such as MTT and CCK8.

Clonogenic Assay Soft Agar Colony formation Assay / Limiting Dilation Assay
Monolayer Cell Proliferation Assay

Colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a single colony. This is considered as the most stringent assay for assaying tumoriogenicity of certain cell types and chemoresistance against drug treatments. Especially, it is the most useful method to identify the cancer stem cell population. We are able to provide a soft-agar colony formation assay and limiting dilution assay.

Angiogenesis assay

Introduction

Angiogenesis, or the formation of new blood vessels, is an important process that plays a significant role in cancer. It is an attractive target for cancer therapeutics, as inhibition of angiogenesis has been proven an effective means to treat solid tumors. Because angiogenic tube formation is a morphological change, cellular imaging-based assays can provide a wealth of information on the degree and quality of angiogenic tube formation.

Results
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